Fig. 4.

DJK-5 suppressed Gram-negative abscess lesion formation. (a) Mice were injected with 4 mg/kg of DJK-5 or saline (control) via intraperitoneal injection prior to subcutaneous infection with P. aeruginosa LESB58. Abscess lesion sizes were measured using a caliper after 72 h, n = 11/group. (b) Bacteria were recovered from saline and DJK-5 IP-treated animals and plated for enumeration three days post-infection, n = 11/group. (c) Mice were infected subcutaneously with P. aeruginosa LESB58 and then treated 2 h later with 4 mg/kg of DJK-5 via intra-abscess injection. Lesions were measured 72 h post-infection with a caliper, n = 10/group. (d) Bacteria were recovered from saline or DJK-5 intra-abscess treated animals and plated for enumeration, n = 10/group. All comparisons were made using unpaired t tests; *, p < 0.05; **, p < 0.01; ***, p < 0.001. All experiments were performed in triplicate.