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Type I HBECs plated and cultured in MSU-1 medium (a) formed colonies with smooth and restricted boundaries, the cells in colonies were contiguous and variable-shaped, and Type I HBECs grown in K-NAC medium (b) were dispersed. (c) Proliferation potential of Type I HBECs cultured in K-NAC medium: cpdl = 38.5, 42.8, and 22.4, respectively, for HBEC30, HBEC31, and HBEC35.
