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Gap junctional intercellular communication of Type I HBECs cultured in K-NAC medium studied by the Lucifer Yellow scrape loading/dye transfer technique. The results show that Type I HBECs in early and middle passage (cpdl = 1, 18, and 24) (a–c) were deficient in dye transfer. In later passage (cpdl 29) (d), the Type I HBECs showed very limited dye transfer. The same area of cells was observed under a phase contrast (left) or fluorescence (right) microscope.
