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Two types of HBECs (Type I HBECs developed in the K-NAC medium and Type II HBECs in 1 : 2 ratio) were inoculated in a poly-D-lysine coated plate. (a, b) After plating for 14 days, these cells formed mammary organoids. (c) After incubation for 28 days, these organoids were found to develop many mammary ductal and terminal end bud-like structures.
