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. 2016 Oct 25;10:247. doi: 10.3389/fncel.2016.00247

FIGURE 9.

FIGURE 9

Barium sensitive potassium channels are required for eEF2K-inhibition mediated fEPSP potentiation. (A) The graph indicates the fEPSP slopes over time under three experimental conditions (control: white circles, n = 4; 5 μM A-484954: black circles, n = 4; 2 mM CsCl2: gray circles, n = 4). (B) Blockage of TEA (10 mM, gray circles)-sensitive potassium channels partially but significantly attenuated A-484954-mediated fEPSP potentiation (black circles, n = 4). The remaining fEPSP potentiation was still significantly different from that of drug-free experiments (white circles, n = 4). (C) Pre-application with 400 μM BaCl2 significantly prevented A-484954-mediated fEPSP potentiation (p < 0.01). fEPSP slopes were similar in size to those with drug-free experiments (control: white circles, n = 4). Horizontal black and gray lines indicate A-484954 and, CsCl, TEA, or BaCl2 application times, respectively. Brackets enclose the time points of significant differences (∗p < 0.05; ∗∗p < 0.01) between groups (circle) per time point. (D) Representative fEPSP traces for the time points 1 and 2 and compounds are depicted. Scale bars: 0.5 mV/10 ms. (E) The bar graph summarizes the observations and significance levels (brackets; ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001) for different experimental conditions in comparison with drug-free (control: white bar) experiments for fEPSP slope values at the 40-min time point.