Figure 4. Clustering of similarly tuned cells in specific minicolumns.

(a–e) Representative examples of minicolumns with various similarity. Tilting angles of black bars represent preferred orientations of individual neurons within each minicolumn. (f,g) The histograms of P_similar (f) and meanΔori (g) of minicolumns. red: experimental data, black: randomized data. *P<0.05, ^***P<0.001, n=50 volumes including layers 2–5 from 30 mice, Mann–Whitney U-test with the Bonferroni correction. (h,i) P_similar of minicolumns in the FOV from experimental data (h) and randomized data (i). Each circle indicates the location of minicolumn and its value of P_similar with colour. Scale bars 30 μm.