FIGURE 2.

Schematic of binary vector pSIM2170 that contains forward and reverse TALENs. Two TALENs were driven by either 35s or FMV constitutive promoter followed by either OCS or Nos terminator, respectively. Each TALEN has a SV40 nuclear localization sequence (NLS), designed repeat variable diresidue (RVD) (E3 or E4 repeat), and truncated C-terminal fused to a FokI cleavage domain. A potato Ubi3 promoter-driven ipt gene cassette was put on the left side of T-DNA and the T-DNA LB was removed in this vector.