FIGURE 4.

Design of knock-in donor vector. The donor vector pSIM2370 T-DNA contains a StUbi7 promoter driven nptII gene cassette at the LB side, and a promoter-less mStALS gene at the RB side. At the sequence level, next to the RB, are sequences of a partial Ubi7 5′ intron region (light gray highlighted), Ubi7 monomer (dark gray highlighted) fused to the mStALS coding sequence (only partial sequence is shown in this figure). The intron sequence contains a spacer between the two TALEN binding sites and the second TALEN recognition site (underlined). There is no ipt expression cassette on the backbone of donor vector.