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. 2016 Oct 25;7:1572. doi: 10.3389/fpls.2016.01572

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Copyright © 2016 Forsyth, Weeks, Richael and Duan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Sequence of LB side flanking region. A pair of primers, one within the endogenous Ubi7 intron and the other within the T-DNA region of the donor vector close to the left border (as shown in Figure 5), were used to amplify targeted insertion in the LB junctions. Amplified products were cloned and sequenced. Sequences of five lines are shown here and aligned to deduce the LB junction after targeted integration. The LB sequence is green highlighted in the deduced sequence with the last three nucleotides red-highlighted, which are lost in the perfect LB cleavage events.