Fig. 4.

Uptake of AdV in DC is blocked by inhibitors of actin-dependent endocytosis. ALDC and 293 cells were cultured with AdV5-Fluo for 1 h at 37 °C in the presence of various endocytosis inhibitors as described in Methods. Extracellular fluorescein was then quenched with trypan blue and fluorescence was measured by flow cytometry. (a) Representative histograms showing fluorescence after various treatments. The markers indicate percentage of fluorescein-positive cells above background. (b) Bar graphs showing means and standard deviations of the percentage of fluorescein⁺ ALDC obtained from six different animals and tested in duplicate or 293 cells tested in triplicate. Asterisks indicate *P<0.05 and **P<0.005 compared to treatment with DMSO.