Figure 1.

Phylogenetic analysis and amino acid residue alignment of soybean cystatins. (A) Phylogenetic analysis of soybean cystatins. Phylogenetic tree construction of soybean cystatins is based on the full-length deduced amino acid sequences using MEGA 6 by the neighbor-joining method with 1000 bootstrap replicates. The tree shows four major phylogenetic clades (clades I to IV) indicated with braces. (B) Alignments of conserved motifs of different clades of the soybean cystatins. The main cystatin conserved motifs in four phylogenetic clades (clades I to IV) are in orange, red, green, and blue boxes.