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. 2016 Oct 25;7:1595. doi: 10.3389/fpls.2016.01595

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Copyright © 2016 Yuan, Li, Wang, Chen, Zhang, Chen, Hao, Shan, Zhang, Chen, Yang, Qiu and Zhou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression profile of cystatins family genes in soybean nodules at five important nodule development time points. Three biological replica samples and three replicate reactions per sample were used to calculate the expression level of each cystatin during the nodule development. Relative expression levels of each cystatin gene were obtained by qPCR and normalized to the average expression level of soybean reference gene QACT, and calculated using the expression level in 12 dN as a starting point control. Data represent the mean ± SE of nine independent replicate experiments. “★”and “★★” indicate statistical difference between different nodule development time points (t-test, p < 0.05 or p < 0.01, respectively). “★”and “★★” without wire frame marker in (G), indicate statistical difference compared to the 12 dN (starting point control). (A) GmCYS2. (B) GmCYS3. (C) GmCYS9. (D) GmCYS10. (E) GmCYS12. (F) GmCYS14. (G) GmCYS15. (H) GmCYS16. (I) GmCYS17. (J) GmCYS18. (K) GmCYS20.