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. 2016 Oct 25;7:1595. doi: 10.3389/fpls.2016.01595

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Copyright © 2016 Yuan, Li, Wang, Chen, Zhang, Chen, Hao, Shan, Zhang, Chen, Yang, Qiu and Zhou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of Glyma.15G227500 overexpression on symbiosis in L. japonicus. (A) Symbiotic phenotypes of transgenic plants at 32 days of post inoculation with M. loti. MAFF303099. Hairy roots expressing vector pU1301 served as a control. Independent transgenic plants were chosen for photography. Bars = 5 mm. (B) Nodule numbers of transgenic hairy roots with altered Glyma.15G227500 transcript levels. Transgenic hairy roots expressing vector (pU1301) served as control. (A) Mean numbers of nodules per plant with standard deviation (SD) of L. japonicus expressing pMUb: Glyma.15G227500 (Gm.15G227500-OX) or the empty vector pU1301 (CK) at 32 days of post inoculation with M. loti. (B) Plants were divided into different groups on the basis of nodules per plant, and the relative ratios of various groups were calculated. (C) Semi-quantitative RT-PCR analysis of the transcript levels of Glyma.15G227500 in the control and Gm.15G227500-OX hairy roots. (D) qPCR analysis of the transcript levels of NIN, Enod40, and Lb in the control and Gm.15G227500-OX hairy roots. Total RNA isolated from root system was used for qPCR. Relative expression levels of NIN, Enod40, and Lb transcripts in Gm.15G227500-OX hairy roots were calculated with reference to that of the control hairy roots.