Skip to main content
. 2016 Apr 11;35(42):5501–5514. doi: 10.1038/onc.2016.90

Figure 9.

Figure 9

Hypermethylation mediates miR-506 silencing in pancreatic cancer. (a) The DNA methylation levels of site five were detected in 13 paired pancreatic cancer tissues and adjacent non-cancer tissues by pyrosequencing. The differences in DNA methylation levels between cancer tissues and adjacent non-cancer tissues were calculated using Student's t-test. Aj: adjacent non-cancerous tissue; T: pancreatic cancer. (b) The DNA methylation levels of site five were detected in 10 normal pancreatic tissues and 45 cancer tissues by pyrosequencing. N: normal pancreas. (c) The association between miR-506 expression levels and the DNA methylation status of miR-506 was evaluated using a Spearman's correlation analysis. (d) Representative output of bisulfite pyrosequencing. (e) Pancreatic cancer cell lines were treated with 5-aza-CdR (3 μM) for 72 h, and miR-506 expression levels were determined via qRT−PCR and normalized to U6. The results are presented as the means±s.d. of the values obtained in three independent experiments. *P<0.05; **P<0.01. (f) The impact of miR-506 DNA methylation status on overall survival in pancreatic cancer patients. The low and high levels of miR-506 methylation are separated according to the median value. The P-values were determined using a log-rank test.