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. 2016 Oct 24;49:42. doi: 10.1186/s40659-016-0103-6

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — Calcium (Ca²⁺) and potassium (K⁺) flux, caspase activity, and gene expression. a Ca²⁺ and K⁺ flux was measured before and after treatment of Siberian tiger fibroblasts with 4.8 μM Cd²⁺ by using the ion flux estimate technique. Statistically significant differences compared to the corresponding controls are denoted by an asterisk (P < 0.05) and a double asterisk (P < 0.01; n = 3). b Activities of caspase-3, -8, and -9 were determined in cytosolic extracts of Siberian tiger fibroblasts. c, d Expression levels of the indicated mRNAs were determined via reverse transcription-polymerase chain reaction 24 h after exposure to Cd²⁺ and normalized to glyceraldehyde-3-phosphate dehydrogenase mRNA levels