Table 2.
Summary of the documented induction/repression conditions for the S. cerevisiae promoters chosen for the GFP-reporter constructs
| Promoter | Name/function | Signaling pathway | Induced/derepressed by | Repressed by | References |
|---|---|---|---|---|---|
| HXT1 | Low-affinity hexose transporter | Snf3p/Rgt2p | High glucose (4% w/v)a | Low glucose (0.1% w/v) | [20] |
| HXT2 | High-affinity hexose transporter | Snf3p/Rgt2p | Low glucose (0.1% w/v) | High glucose (4% w/v) | [20] |
| HXT4 | High-affinity hexose transporter | Snf3p/Rgt2p | Low glucose (0.1% w/v) | High glucose (4% w/v); more glucose-repressed than HXT2 | [20, 77] |
| SUC2 | Invertase | SNF1/Mig1p | Low glucose (0.1% w/v) | High glucose (2% w/v) and depleted glucose (0% w/v) | [78] |
| CAT8 | Alternative carbon source response-activator | SNF1/Mig1p | Low glucose (0.2% w/v) | High glucose (4% w/v) | [79] |
| TPS1 | Trehalose-6-phosphate synthase (56 kD subunit) | cAMP/PKA | Glucose limitation, stress conditions (e.g. heat, nutrient starvation, oxidative stress) | High glucose; however, a basal expression level has been observed when growing on rapidly fermentable sugars | [80, 81] |
| TPS2 | Trehalose-6-phosphate synthase (102.8 kD subunit) | cAMP/PKA | |||
| TEF4 | Translation elongation factor | cAMP/PKA | – | Stress conditions | [82, 83] |
aGlucose 4% (w/v) corresponds to 40 g/l