Fig. 7.

ITPR1 is expressed in a subset of carcinomas of the lung. a Quantitative Western blot analysis showing ITPR1 expression as percentage of the ITPR1 expression in normal human bronchial epithelial (NHBE) cells, in the small cell lung cancer cell line H1339 and in the non-small cell lung cancer cell line HCC. The expression of IP3R was increased with H1339 showing the highest expression (n = 4, *P < 0.01 versus all other groups). Image reproduced from Bergner et al. (2009) [19] (distributed under the terms of the Creative Commons Attribution License [http://creativecommons.org/licenses/by/2.0]). b–d Indirect immunofluorescence-based immunohistochemistry assay showing ITPR1 expression within adenocarcinoma tissue obtained from patient 1 (b binding of a commercial rabbit antibody to ITPR1 visualized using a goat anti-rabbit IgG Alexa Fluor® 568-labelled secondary antibody [red fluorescence]; c binding of patient serum IgG visualized by use of a goat anti-human IgG secondary antibody labelled with Alexa Fluor® 488 [green fluorescence]; d staining of cell nuclei by DAPI [blue fluorescence])