Fig. 1.

Changes in intracellular cytokine production in T cell subsets during pollen season. Isolated PBMCs were stimulated with leukocyte activation cocktail and were cultured for 4 h. After this time, intracellular production of cytokines was detected in CD4+ T cells (a) and in CD8+ T cells (b). IL-4 positive or IL-17A positive T cells (a) and IFNγ positive or TNF positive T cells (a and b) are shown. V1—before allergy season, V2—during allergy season immediately following the appearance of symptoms, V3—2 weeks after onset of symptoms, CTRL—control group; data are shown as mean ± SEM; *P < 0.05  in paired samples t test; ^# P < 0.05 in unpaired samples t test; ^$ P < 0.05 in repeated measures ANOVA, testing all three patients’ visits. Comparisons of cell populations for pairs of single patients’ visits were performed using paired samples t test, while comparisons of cell populations between single patients visit and control subjects were performed using unpaired samples t test