Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Oct 24;16:413. doi: 10.1186/s12906-016-1404-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Fig. 1 — Effect of E. humifusa Willd (EA/EuH) extracts on TNFα-induced IKK phosphorylation. Serum-starved MDA-MB-231 cells were left untreated or treated with n-hexane (Hex), chloroform (CH), ethylacetate (EA), n-butanol (Bu), or aqueous (H₂O) fraction (each 5 μg/mL) for 30 min before stimulation with TNFα (10 ng/mL). After 60 min, whole-cell lysates were prepared, and immunoblotting was performed using the phospho-specific antibody against IKKα/β (Ser176/181). The anti-GAPDH antibody was used as an internal control. Band intensities were analyzed using the ImageJ software. TNFα alone treated-band intensities were set to 100 % (control). The relative values of % of control were plotted as the mean ± SD. P values were analyzed by Sidak’s test. (n = 3)