Fig. 5.

Mtb antigen induced single, duo and polyfunctional cytokine producing CD4+ T cells. PBMCs were obtained from patients with active TB prior to treatment and left unstimulated or stimulated with ESAT- 6 or Ag85 without (open boxes) or with indomethacin (hatched boxes) after 12 h (a) and 36 h (b) stimulation (n = 18 and n = 17 respectively). Boolean gating strategy was used to create cytokine combinations of single-producing, duo-producing and polyfunctional T cells. P- values were calculated by Wilcoxon matched pairs test ( *p <0.05, **p < 0.01). Plots are shown as box plots with median, IQR and minimum/maximum values