Figure 3. PBP1A dynamics and activity are regulated by LpoA in vivo.

Apparent diffusion constants of PAmCherry-PBP1A molecules (orange) imaged in a strain deleted for lpoA (TKL242) and treated with cefsulodin, mecillinam, or ampicillin (100 μg ml⁻¹ each). Diffusion constants of PAmCherry-PBP1B molecules (blue) were determined with and without lpoB (strain TKL243). Error bars indicate the s.d. of 1,000 bootstrap samples.