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MALDI-TOF/TOF-MS/MS analysis of the cross-linking product with BS3 of laminin β1 LN domain (amino acis194-200 connected with amino acids 207-217; [M+H]+exp 2282.322), lysines at positions 198 and 212 were found to be connected (marked with asterisks). Upper panel: the identified fragment ions are shown in bold; lower panel: tandem MS (MS/MS); the insets show the y6 fragment ion and the precursor ion, both exhibiting the characteristic D0/D4 isotope pattern.
