Table 1.
Intramolecular cross-linked products of isolated laminin β1 and γ1 LN domains using BS3 as cross-linker; all cross-linked products were verified by MS/MS data. The cross-linked amino acids are printed in italics and are underlined. The cross-linked product presented in Figure 4 is highlighted.
| Protein | MH+exp | MH+theo | Sequence | Amino acids | Δm [ppm] |
MS/MS | D0/D4 | Digestion |
|---|---|---|---|---|---|---|---|---|
| β1 | 1293.794 1297.821 |
1293.793 1297.818 |
218–227 | R/IKFVKLHTLG/D | 1 2 |
b5–9 y4–5 |
1.9 | In-solution |
| β1 | 1293.786 1297.810 |
1293.793 1297.818 |
218–227 | R/IKFVKLHTLG/D | 5 6 |
b5–9 y3–5 |
1.8 | In-gel |
| β1 | 1672.824 1676.846 |
1672.822 1676.847 |
194–206 | L.DPAFKIEDPYSPR.I | 1 1 |
y1,y12 b3 |
1.4 | In-solution |
| β1 | 1672.804 1676.830 |
1672.822 1676.847 |
194–206 | L.DPAFKIEDPYSPR.I | 11 10 |
y1–3,y12; b2,b4 |
1.4 | In-gel |
| β1 | 1856.951 1860.976 |
1856.943 1860.968 |
192–206 | R.ALDPAFKIEDPYSPR.I | 4 4 |
y1–3;y12, b2 | 1.4 | In-solution |
| β1 | 2282.310 2286.329 |
2282.334 2286.359 |
194–200 + 207–217 |
L.DPAFKIE.D + R.IQNLLKITNLR.I |
11 13 |
1: b3,y6 2: y1,2;b2,3 |
1.6 | In-solution |
| β1 | 2466.415 2470.444 |
2466.433 2470.458 |
192–200 +207–217 |
R.ALDPAFKIE.D +R.IQNLLKITNLR.I |
7 6 |
2: b1,3;y1–4 | 1.5 | In-gel |
| β1 | 2466.455 2470.479 |
2466.433 2470.458 |
192–200 +207–217 |
R.ALDPAFKIE.D +R.IQNLLKITNLR. |
9 9 |
1:b2,3 2:b2 |
1.6 | In-solution |
| γ1 | 2118.135 2122.156 |
2118.149 2122.174 |
128–143 | R.LKFHTSRPESFAIYKR.T | 7 8 |
y1,2,5–7,9 b7,14,15 |
1.8 | In-solution |
| γ1 | 2118.145 2122.166 |
2118.149 2122.174 |
128–143 | R.LKFHTSRPESFAIYKR.T | 2 4 |
y1, b15 | 1.7 | In-gel |
| γ1 | 2651.284 2655.307 |
2651.299 2655.324 |
189 – 212 | S.DISPLTGGNVAFSTLEGRPSAYNF.D | 6 6 |
y1,2,6– 12,14,18,21,23 b2–4,6,9–11,18 |
1.3 | In-gel |
| γ1 | 2651.286 2655.301 |
2651.299 2655.324 |
189 – 212 | S.DISPLTGGNVAFSTLEGRPSAYNF.D | 5 9 |
y2,6,23; b2,3,6 | 1.5 | In-solution |