Table 2.
Intramolecular cross-linked products of cross-linking reaction mixtures containing both laminin β1 and γ1 LN domains; the cross-linker (BS2G or BS3) is indicated. All cross-linked products were verified by MS/MS data. The cross-linked amino acids are printed in italics and are underlined.
| Laminin β1 | |||||||
|---|---|---|---|---|---|---|---|
| MH+exp | MH+theo | Sequence | Amino acids and cross-linker | Δm [ppm] |
MS/MS | D0/D4 | Digestion |
| 1293.787 1297.812 |
1293.793 1297.818 |
218–227 | R.IKFVKLHTLG.D + BS3 | 5 5 |
y4–5; b5–9 |
1.3 | In-solution |
| 1293.821 1297.841 |
1293.793 1297.818 |
218–227 | R.IKFVKLHTLG.D + BS3 | 22 18 |
y3-5; b5–9 | 1.8 | In-gel |
| 1672.823 1676.847 |
1672.822 1676.847 |
194–206 | L.DPAFKIEDPYSPR.I + BS3 | 1 0 |
b3, y1–3,12 | 1.4 | In-solution |
| 1672.833 1676.860 |
1672.822 1676.847 |
194–206 | L.DPAFKIEDPYSPR.I + BS3 | 7 8 |
b3; y1–3,9,12 | 3.0 | In-gel |
| 2282.322 2286.342 |
2282.312 2286.337 |
194-200+ 207-217 |
L.DPAFKIE.D +R.IQNLLKITNLR.I + BS3 |
4 2 |
1 :b3 2 :b2–4; y1–5, |
1.8 | In-gel |
| 2466.430 2470.454 |
2466.433 2470.458 |
192-200+ 207–217 |
R.IQNLLKITNLR.I + R.ALDPAFKIE.D + BS3 |
1 2 |
- | 1.6 | In-gel |
| 1421.842 1425.867 |
1421.847 1425.869 |
207–217 | R.IQNLLKITNLR.I + BS2G | 4 1 |
y1–5, b1–5 | 0.8 | In-solution |
| 1630.800 1634.793 |
1630.775 1634.800 |
194–206 | L.DPAFKIEDPYSPR.I + BS2G | 15 4 |
y1,2,12 | 0.6 | In-gel |
| 1836.901 1840.924 |
1836.865 1840.890 |
176–191 | R.YSDIEPSTEGEVIFR.A amidated(C-term);+ BS2G | 20 18 |
y1–8 | 0.7 | In-solution |
| 1836.921 1840.944 |
1836.865 1840.890 |
176–191 | R.YSDIEPSTEGEVIFR.A amidated (C-term); + BS2G | 30 29 |
y1–4 | 0.6 | In-gel |
| 1935.877 1939.900 |
1935.883 1939.908 |
152–168 | Y.DCESSFPGISTGPMKKV.D + BS2G | 3 4 |
y2–3,5–8,10–13, b4, b9, b6, b4 |
0.7 | In-gel |
| 2240.265 2244.286 |
2240.265 2244.290 |
194–200+ 207–217 |
L.DPAFKIE.D + R.IQNLLKITNLR.I + BS2G |
0 2 |
b3, y1,4,17 |
0.7 | In-solution |
| 2240.260 2244.285 |
2240.265 2244.290 |
194–200+ 207–217 |
L.DPAFKIE.D + R.IQNLLKITNLR.I + BS2G |
2 2 |
1: y1–5 ; b2–5 2 : b2–4 |
0.7 | In-gel |
| 2501.238 2505.257 |
2501.235 2505.260 |
136–147+ 192–200 |
R.SSDFGKTWGVYR.Y+R.ALDPAFKIE.D + BS2G | 1 1 |
1 : b2, y1,2,5 | 0.5 | In-solution |
| 2501.216 2505.239 |
2501.235 2505.260 |
136–147+ 192–200 |
R.SSDFGKTWGVYR.Y+R.ALDPAFKIE.D + BS2G | 8 8 |
A: b2,3,5; y1,4–6 b : b3 |
0.6 | In-gel |
| 2552.132 2556.149 |
2552.135 2556.160 |
152–173 | Y.DCESSFPGISTGPMKKVDDIIC.D + BS2G | 1 4 |
b6,9,10,17,21 y10,11,13,17 |
0.5 | In-solution |
| 2552.021 2556.044 |
2552.135 2556.160 |
152–173 | Y.DCESSFPGISTGPMKKVDDIIC.D + BS2G | 45 45 |
- | 0.6 | In-gel |
| 2751.278 2755.315 |
2751.312 2755.337 |
33–55 | K.LSVTSTCGLHKPEPYCIVSHLQE.D + BS2G | 12 8 |
y3–y6,10–14 b2,4,10,11 |
0.5 | In-solution |
| 2751.272 2755.312 |
2751.312 2755.337 |
33–55 | K.LSVTSTCGLHKPEPYCIVSHLQE.D + BS2G | 15 9 |
b2,4,10 y5–6,10,12 |
0.6 | In-gel |
| 3078.499 3082.529 |
3078.502 3082.527 |
30–55 | R.AQKLSVTSTCGLHKPEPYCIVSHLQE.D + BS2G | 1 1 |
b15,17 y4,5,10,12 |
0.3 | In-gel |
| Laminin γ1 | |||||||
|---|---|---|---|---|---|---|---|
| MH+exp | MH+theo | Sequence | Amino acids and cross-linker | Δm [ppm] |
D0/D4 | MS/MS | Digestion |
| 2118.151 2122.176 |
2118.149 2122.174 |
128–143 | R.LKFHTSRPESFAIYKR.T + BS3 | 1 1 |
1.3 | b7,9,15 y1,7,9 |
In-solution |
| 2118.172 2122.170 |
2118.149 2122.174 |
128–143 | R.LKFHTSRPESFAIYKR.T + BS3 | 11 2 |
0.8 | (y1,b15) | In-gel |
| 2076.080 2080.125 |
2076.103 2080.128 |
128–143 | R.LKFHTSRPESFAIYKR.T + BS2G | 11 1 |
0.8 | b1,15 y1,6,7,10 |
In-solution |
| 2852.190 2856.198 |
2852.226 2856.251 |
147–169 | E.DGPWIPYQYYSGSCENTYSKANR.G + BS2G | 13 19 |
0.4 | y1-3; b3– b5, b8, b16 |
In-solution |
| 2949.432 2953.455 |
2949.431 2953.456 |
239–263 | G.DDVFNEPKVLKSYYYAISDFAVGGR.C + BS2G | 0 0 |
0.7 | b18,b20, y1,3,19 |
In-solution |