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Im7 binding of truncated NColE7 mutants. The result of the GST pull‐down assay showing the interaction of Im7 with N‐terminally truncated NColE7 mutants in their GST‐tagged forms. The first lane contains the Thermo Scientific™ Unstained Protein Molecular Weight Marker. In lanes 3–5 the NColE7 proteins were shown to bind Im7 under the conditions of the GST‐affinity purification, while the shortest mutant protein in lane 2 did not.
