Figure 2.

Side effects of the pLysSRARE2 plasmid may lead to the false conclusion that the tRNA genes stimulate expression. A: Whole cell fluorescence (arbitrary units)/OD_600nm) from E. coli BL21 (DE3) expressing CYP720B4 fused with gfp and B: BRCA1 translationally coupled with the mCherry red fluorescent protein. In both cases expression are in the presence of pLysSRARE2, pLysS or derivatives were either the 3.5 gene or the extra tRNA genes were deleted. C) Semi‐quantitative immunoblot detection of T7 lysozyme from BL21 (DE3) harboring the pLysSRARE2 or the pLysS plasmids or derivatives were either the 3.5 gene or the extra tRNA genes were deleted. D) Sense (dark grey) and anti‐sense (white) read counts from RNA originating from the tRNA regions in the pLysSRARE2 plasmid isolated from BL21 (DE3) harboring the plasmid.