Table 1.
Summary of the SEC‐MALS and DSC analysis of the ProCat constructs
| Theoretical Mass (kDa) | SEC‐MALS Mass (kDa) | T m (˚C) | ΔT m a (˚C) | |
|---|---|---|---|---|
| ProCat(WT)processed | 45.2 | 47.4 ± 0.2 | 51.9 ± 0.1 | 1.6 |
| ProCat(Q152H)zymogen | 46.3 | 48.5 ± 2.0 | 52.8 ± 0.2 | −0.5 |
| ProCat(Q152N)zymogen | 45.2 | 49.0 ± 0.2 | 51.4 ± 0.3 | −0.4 |
| ProCat(Q152N)processed | 45.2 | 46.1 ± 0.7 | 43.5 ± 0.4 | 1.7 |
| ProCat(S386A)zymogen | 47.3 | 49.2 ± 2.3 | 53.2 ± 0.1 | −0.5 |
a
ΔT m represents the difference in melting temperature between the protein in the absence and presence of peptide.