Table 2.
Catalytic Residues of Carboxylic Ester Hydrolase Families with Known Tertiary Structures
| Clan | Family | PDB designation | Producing species and enzyme | Catalytic residues |
|---|---|---|---|---|
| A | 1 | 1QIT | Moorea producens (Lyngbya majuscula) decarboxylating thioesterase | S100/E124/H266 |
| A | 2 | 4CCY | Bacillus subtilus carboxylesterase CesB | S130/E245/H274 |
| A | 3 | 4KRX | Escherichia coli acetyl esterase Aes | S165/D262/H292 |
| A | 4 | 4CG1 | Thermobifida fusca polyethylene terephthalate degrading hydrolase | S130/D176/H208 |
| A | 5 | 2XLB | Bacillus pumilus (Bacillus mesentericus) acetyl xylan esterase | S181/D269/H298 |
| A | 6 | 3C5V | Human PP2A‐specific methylesterase | S156/D181/H349 |
| A | 7 | 3D59 | Human plasma platelet‐activating factor acetylhydrolase | S273/D296/H351 |
| A | 8 | 4G4G | Sporotrichum thermophile (Myceliophthora thermophila) glucuronyl esterase | S213/E236/H346 |
| A | 9 | 1K8Q | Dog gastric lipase | S153/D324/H353 |
| A | 10 | – | – | – |
| A | 11 | 4UYU | Human WNT deacylase notum | S232/D340/H389 |
| A | 12 | 2JGJ | Mouse acetylcholinesterase | S203/E334/H447 |
| A | 13 | 1JJF | Clostridium thermocellum feruloyl esterase | S172/D230/H260 |
| B | 14 | – | – | – |
| B | 15 | 1U8U | Escherichia coli thioesterase/protease/lysophospholipase L1 | S10/D154/H157 |
| B | 16 | 1I6W | Bacillus subtilis lipase | S77/D133/H156 |
| B | 17 | 4PSD | Trichoderma reesei cutinase | S164/D216/H229 |
| B | 18 | 1VYH | Mouse PAF‐AH holoenzyme | S48/D/193/H196 |
| B | 19 | 1DEO | Aspergillus aculeatus rhamnogalacturonan acetylesterase | S9/D192/H195 |
| B | 20 | 1EB8 | Manihot esculenta hydroxynitrile lyase | S80/D208/H236 |
| B | 21 | 4HYQ | Streptomyces albidoflavus phospholipase A1 | S11/H218 |
| B | 22 | 1CVL | Chromobacterium viscosum lipase | S87/D263/H285 |
| B | 23 | 4X92 | Human lysosomal phospholipase A2 | S165/D327/H359 |
| B | 24 | 4FDM | Bacillus L2 lipase | S113/D317/H358 |
| C | 25 | 1DIN | Pseudomonas knackmussi dienelactone hydrolase | C123/D171/H202 |
| C | 26 | 4ETW | Shigella flexneri enzyme/ACP substrate gatekeeper | S82/D207/H235 |
| C | 27 | 3HJU | Human monoglyceride lipase | S121/D239/H269 |
| C | 28 | 3CN9 | Pseudomonas aeruginosa carboxylesterase | S113/D166/H197 |
| C | 29 | 1TQH | Geobacillus stearothermophilus carboxylesterase Est30 | S94/D193/H223 |
| C | 30 | 3BF7 | Escherichia coli esterase | S89/D113/S206/H234 |
| C | 31 | 1LBS | Moesziomyces antarticus triacylglycerol hydrolase | S105/D187/H224 |
| D | 32 | 2DG0 | Staphylococcus aureus lactonase | D138/D236 |
| D | 33 | 1V04 | Human/rabbit/mouse/rat serum paraoxonase | H115/H134 |
| E | 34 | 1PPA | Agkistrodon piscivorus lysine 49 phospholipase A2 | H48/Y73/D99 |
| E | 35 | 1POC | Apis mellifera venom phospholipase A2 | H34/D35 |
| E | 36 | 2WG7 | Rice class X1b phospholipase A2 | H61/D62 |
| – | 37 | 1RZW | Archaeglobus fulgidis peptidyl‐tRNA hydrolase | H20/D93/H113 |
| – | 38 | 3EB9 | Trypanosoma brucei 6‐phosphogluconolactonase | D163/H165 |
| – | 39 | 1RP1 | Dog pancreatic lipase related protein | S152/D176/H263 |
| – | 40 | 1TGL | Rhizomucor michei triacylglycerol lipase | S144/D203/H257 |
| – | 41 | – | – | – |
| – | 42 | 1CJY | Human cytosolic phospholipase A2 | S228/D549 |
| – | 43 | 3KVN | Pseudomonas aeruginosa autotransporter EstA | S14/D286/H289 |
| – | 44 | 1UWC | Aspergillus niger feruloyl esterase | S133/D194/H247 |
| – | 45 | 4HOY | Acinetobacter baumanni peptidyl‐tRNA hydrolase | H22/N70/D95/N116 |
| – | 46 | 1TIA | Penicillium camemberti lipase | S145/D199/H259 |
| – | 47 | 1CHD | Salmonella enterica chemotaxis receptor methylesterase | S164/H190/D286 |
| – | 48 | 4PKB | Solanum cardiophyllum patatin‐17 | S77/D215 |
| – | 49 | 3CL6 | Pseudomonas fluorescens allantoinase | E36/H259 |
| – | 50 | 4DI9 | Sphingomonas paucimobilisi 2‐pyrone‐4,6‐dicarboxylic acid carboxylase | R124/D248 |
| – | 51 | – | – | – |
| – | 52 | 2NSP | Dickeya dadantii (Erwinia chrysanthami) pectin methylesterase | Q177/D178/D199 |
| – | 53 | 2WYM | Escherichia coli l‐ascorbate‐6‐phospholactonase | D121/H122/D226/H281 |
| – | 54 | 3KO9 | Plasmodium falciparum d‐amino acid deacylase | T90 |
| – | 55 | 2ERV | Pseudomonas aeruginosa 3‐O‐deacylase | H126/S128/E140 |
| – | 56 | 1ILZ | Escherichia coli phospholipase A | H142/S144/N156 |
| – | 57 | – | – | – |
| – | 58 | 1CJY | Human cytosolic phospholipase A2 | S228/D549 |
| – | 59 | 2JY9 | Thermus thermophilus YaeJ bound to ribosome | Gln28 |
| – | 60 | 1FLC | Influenza C virus hemagglutinin‐esterase fusion glycoprotein | S57/D352/H355 |
| – | 61 | 2Z8X | Pseudomonas sp. family I.3 lipase | S207/D255/H313 |
| – | 62 | 4NFU | Arabidopsis thaliana signaling node | S123/D187/H317 |
| – | 63 | 3WMT | Aspergillus oryzae feruloyl esterase B | D17/S203/H457 |
| – | 64 | – | – | – |