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. 2016 Aug 24;112(2):543–554. doi: 10.1093/cvr/cvw201

Figure 5.

Figure 5

Knockdown of lncRNAs reduced the expression of ECM genes. (A) qRT-PCR detecting the knockdown of lncRNA expression in isolated mouse CF. The result of each lncRNA knockdown was the combination of the results from two independent siRNAs. Data were obtained from four independent experiments. (B) Immunochemistry detecting the expression of αSMA in lncRNA knockdown CF. DAPI marks the nucleus. Bar=10 µm. αSMA immunostaining signal was quantified and presented as the Relative Staining Intensity (right panel). Data were obtained from three independent experiments. (C) Western blotting of αSMA in lncRNA knockdown CF. β-tubulin serves as a loading control. Molecular weight marker was provided. Relative αSMA protein levels were quantified (lower panel). Data were obtained from three independent experiments. (D) qRT-PCR detecting the expression of marker genes for ECM synthesis, FBN1, and for collagen synthesis, Col3A1, Col8A1 in lncRNA knockdown CF. Each CF isolation was treated as one independent experiment. Data were obtained from four independent experiments. *P <0.05; **P <0.01.