Figure 2.

Hypoxia reporter characterization in vitro. (A) Representative images of GFP MET1-5HRE-ODD-mCherry cells in vitro in normoxia and hypoxia induced by 1% O₂, 0.2 mM CoCl₂ and 90 µM DFOM for 20 hours respectively. (B) Quantification of percent of cells expressing mCherry in normoxia, or after 20 hours of hypoxia induction with 1% O₂, 5% CO₂ and 94% N₂ in a gas chamber or with 0.2 mM CoCl₂ or with 90 µM DFOM. Error bars: mean ± s.e.m. (C) mCherry expression kinetics of MET1 and MDA-MB-231 hypoxia reporter cells upon 1% O₂ induction. Error bars: mean ± s.e.m. (D) mCherry expression in MET1 and (E) MDA-MB-231 hypoxia reporter cells. Images analyzed were taken at the times shown after the cells were returned to normoxia after hypoxia treatment of 1%O₂ or 0.2 mM CoCl₂. error bars: mean ± s.e.m.