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. 2016 Aug 19;214(9):1421–1429. doi: 10.1093/infdis/jiw386

Figure 1.

Figure 1.

Oxacillin minimum inhibitory concentrations (MICs) determined by the Etest (A), relative expression of mecA in the absence (B), and the presence of half MICs of oxacillin (C) and penicillin-binding protein 2a (PBP2a) agglutination (D) of study methicillin-resistant Staphylococcus aureus parental and sarA mutant strain sets. B, Relative transcription levels of mecA represent the mean (+SD) of 3 biological replicates in vitro. The fold changes represent the normalized mecA expression (2−ΔCt) to the housekeeping gene gyrB. *P < .05, compared with their respective parental strains. Open bars, parental strain; shaded bars, sarA mutant. D, mecA-positive (ATCC 43300) and mecA-negative (ATCC 25923) controls. Interpretation of agglutination intensity: +++, high; ++, moderate; +, low; and −, none.