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Biofilm formation (A) and relative expression of sarA (B) and asp23 (C) in the methicillin-resistant Staphylococcus aureus JE2 parental strains and its sarA and/or sigB mutant. Relative transcript levels of sarA and asp23 represent the mean (+SD) of 3 biological replicates in vitro. The fold changes represent normalized target gene expression (2−ΔCt) to the housekeeping gene gyrB. *P < .05, compared with the JE2 parental strain.
