Figure 4.

Copy Number Alteration Analysis. Normalized copy number (CN) ratios for genes commonly deleted or amplified in medulloblastoma. A negative control and the tumor sample were compared by qPCR to a pooled (10 individual) control as a copy number benchmark. The pooled control is normalized to a CN ratio = 1; experimental ratios that drop below 0.7 or rise above 1.3 are considered to be deleted or amplified, respectively. The 0.53 ratio for TP53 in the medulloblastoma correlates with FISH results indicating deletion of distal 17p. No other genes passed cut-off criteria for copy number alteration.