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. 2016 Aug 30;17(10):1079–1088. doi: 10.1080/15384047.2016.1219819

Figure 2.

Figure 2.

Cisplatin treatment triggers increase in rNanog/DsRed cells in vitro. (A) Induction of stem cell factors by cisplatin. Ovc316 cells were treated with 2.5 µM cisplatin for 8 days and analyzed by Western blot. Cisplatin treatment triggered Oct4, Sox2, Nanog upregulation. SMC1 (Structural maintenance of chromosomes protein 1 served as a loading control. (B) Expression cassette of rNanog/DsRed reporter lentivirus vector. DsRed expression is under the control of a minimal (m) CMV promoter and tandem repeats of the Nanog transcriptional response element. The zeocin resistance gene is under the control of the ubiquitously active phosphoglycerate kinase (PGK) promoter. ψ: lentivirus packaging signal. C-E) Characterization of a representative xenograft-derived cell culture. (C) Bright-field and rNanog/DsRed expression in ovc-rNanog/DsRed cells. (D) Flow cytometry analysis for rNanog/DsRed and E-cadherin. (E) Primary ovarian cancer cells expressing DsRed in tumor spheres. (F) rNanog/DsRed expression before and after treatment with 2.5 μM cisplatin for 8 days (added only once). A representative study is shown. The red peak represents DsRed negative ovc316 cells.