Figure 5.

Induction of MAPK, a pathway involved in EMT of ovc-MAPK cells after treatment with cisplatin and small-molecule compounds. (A) Expression cassette of SRE/MAPK-luc reporter lentivirus vector. Luciferase expression is under control of a minimal CMV promoter and tandem repeats of the SRE transcriptional response element (SRE-TATA). hPGK: human phosphoglycerate kinase promoter, PuroR: puromycin resistance gene, cppt: Central polypurine tract. (B) Luciferase expression in ovc-MAPK cells that were modified with the SRE/MAPK-reporter lentivirus vector. Cells were treated for 4 days with 2.5 µM cisplatin alone (second bar) and with cisplatin in combination with small-molecule compounds at a final concentration of 2 μM. DMSO is the solvent of and was used as a negative control (first bar). EGF was used as a trigger of EMT at a concentration of (100 ng/ml) (third bar). Luciferase activity was normalized to total protein concentration and expressed as RLU/mg protein. N = 3, * p < 0.05.