Figure 6.

Screening of small-molecule EMT/MET-regulatory compounds for ability to block cisplatin triggered increase in rNanog/DsRed cells. Ovc-rNanog/DsRed cells were incubated without or with 2.5 μM cisplatin for 4 days in the presence or absence of the indicated compounds at the final concentration of 10 μM. Shown is the percentage of rNanog/DsRed-positive cells. “Control” was set as 100%. DMSO is the solvent for the compounds and is therefore used as a control. The goal was to find compounds that would reduce cisplatin-triggered CSC formation (“w/ Cis”) (upper red line) back to the control level (lower red line) without exerting a significant effect on its own in the absence of cisplatin (“w/o Cis”). N = 3.