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. 2016 Mar 22;10(5):505–515. doi: 10.1080/19336918.2016.1163453

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© 2016 The Author(s). Published with license by Taylor & Francis.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License http://creativecommons.org/licenses/by-nc/3.0/, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 3. — α5 and αvβ3 clusters in U2OS cells adhering to nanopatterned surfaces functionalized with α5β1 and αvβ3 integrin selective ligands. (A) Cells adhering to surfaces with 30 nm interparticle spacing; and (B) Cells adhering to surfaces with 60 nm particle spacing. Upper row: Cells adhering to α5β1 integrin selective ligands. Lower row: Cells adhering to αvβ3 integrin selective ligands. Left: Staining for α5 clusters. Middle: Staining for αvβ3 clusters. Right: Lookup table displaying the colocalization of α5 and αvβ3 integrin clusters (pixel with positive signals for both integrins are shown in yellow).