Table 1.
CPA solution | |||||||
---|---|---|---|---|---|---|---|
Permeating CPA (%, v/v) | |||||||
Group | EG | DMSO | PROH | Trehalose (mol/L) | CPA concentration | Cryodevice | Method |
VSQC-ED | 8% | 8% | 1 | Low | QC | Vitrification | |
SFSCSLC-ED | 8% | 8% | 1 | Low | CS | Slow freezing | |
VSCSLC-ED | 8% | 8% | 1 | Low | CS | Vitrification | |
VSCSLC-EP | 8% | 8% | 1 | Low | CS | Vitrification | |
VSCSLC-PD | 8% | 8% | 1 | Low | CS | Vitrification | |
VSCSHC-ED | 15% | 15% | 0.5 | High | CS | Vitrification | |
VSCSHC-EP | 15% | 15% | 0.5 | High | CS | Vitrification | |
VSCSHC-PD | 15% | 15% | 0.5 | High | CS | Vitrification |
CPA, cryoprotective agent; CS, conventional straw; DMSO, dimethyl sulfoxide; EG, ethylene glycol; PROH, 1,2-propanediol; QC, quartz capillary.