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. Author manuscript; available in PMC: 2016 Oct 25.
Published in final edited form as: Cell Rep. 2016 Sep 27;17(1):11–18. doi: 10.1016/j.celrep.2016.08.085

Figure 1. Nonenzymatic dipeptide hydrolysis and aminolysis.

Figure 1

(A) Representative electrophoretic TLC of a time course of nonenzymatic hydrolysis and aminolysis from free fMet-Phe peptidyl tRNA.

(B) Quantification of data in A. Fractional radioactivity corresponding to the released product was plotted against time and fit to one-phase exponential association equation.

(C) Dipeptide release rates calculated for hydrolysis of free peptidyl-tRNA.

(D) Dipeptide release rates calculated for aminolysis of free peptidyl-tRNA. Note the general similarity between hydrolysis and aminolysis rates.

(E) Dipeptide release rates calculated for nonenzymatic hydrolysis of ribosomal peptidyl-tRNA. The error bars represent the standard error obtained from the nonlinear regression. Most of the reactions were done in duplicates and the deviation was less than 20 % between the observed rates.