Fig. 2.

Summary of salient findings in conditional deletion of Pkd1 and Pdk2 in osteoblasts. Pkd1^cko mice had decreased bone volume, formation and resorption accompanied by increased bone marrow fat, whereas Pkd2^cko also had low turnover osteopenia, but with decreased marrow fat. Oil-Red-O staining of decalcified femur sections and µCT analyses of OsO₄ stained decalcified tibias showed that the numbers of fat droplets and adipocytes were greater in both Pkd1^{Col1a1(3.6)-cko} and Pkd1^Oc-cko mice compared with control mice. PPARγ and adipocyte markers, including Lpl (lipoprotein lipase) and aP2 (adipocyte fatty acid-binding protein were significantly increased in the femurs of Pkd1-deficient mice in a Pkd1 gene dosage-related manner. Primary Obs derived from Pkd1^{Col1a1(3.6)-cko}, Pkd1^Oc-cKO, and Pkd1^Dmp1-cKO mice exhibited increased adipogenesis