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. Author manuscript; available in PMC: 2017 Oct 10.
Published in final edited form as: Cancer Cell. 2016 Sep 22;30(4):623–636. doi: 10.1016/j.ccell.2016.08.015

Figure 1.

Figure 1

Establishment and optimization of FP-HTS assay for selection of reagents and small-molecule compounds targeting MDM2 protein-XIAP IRES interaction (A) Detection of fluorescence for XIAP IRES RNA labeled with fluorescein. (B) FP assay for interaction between fluorescence-labeled XIAP IRES and MDM2 RING protein, data represent mean ± SD of three independent experiments. (C) Signal-to-noise (S/N) ratios and Z′ factors of the assay were obtained for estimating suitability of FP assay used for HTS. (D–G) HTS using optimized FP assay in four libraries, as indicated. The mP values for each well containing library agent were recorded. Percentage of inhibition was calculated to identify hits, as indicated on graph for each library, by setting activity cutoff at 50–70% inhibition and fluorescence intensity (FI) value of vehicle control at <1.5–2. See also Figure S1.