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. Author manuscript; available in PMC: 2017 Oct 10.
Published in final edited form as: Cancer Cell. 2016 Sep 22;30(4):623–636. doi: 10.1016/j.ccell.2016.08.015

Figure 2.

Figure 2

Cell-based assay to select lead compounds (leads) inhibiting MDM2 and XIAP expression in cancer cells (A) Representative Western blots show MDM2 and XIAP expression in EU-1 ALL cells, when treated for 24 hr with promising hits selected from libraries as indicated. Dose of each hit used for cell treatment was equal to the IC50 value for fluorescence inhibition in HTS (data not shown). Structures of 8 leads inhibiting both MDM2 and XIAP were shown. (B) Western blot for MDM2 and XIAP expression in ALL (EU-1 and EU-3) and NB (SH-EP1 and NB-1691) cell lines having MDM2 overexpression, C, control; T, treatment. (C and D) Western blot for time-course (C) and dose-response (D) of MDM2 and XIAP inhibition in EU-1 cells treated with MX3 using indicated conditions. (E) Similar Western blot assays for MDM2 and XIAP expression in EU-1 cells treated with two additional leads (MX11 and MX69). See also Figure S2.