Table 1.

PCR primers used to assay immediate-early gene, vasopressin and EGFP mRNAs.

Primers	Sequence	TA *	TM (°C) *
Rat c-fos mRNA primer pairs	5′-AGCATGGGCTCCCCTGTCA (forward) 5′-GAGACCAGAGTGGGCTGCA (reverse)	60 °C, 30 s	83.4
Rat NGFI-A mRNA primer pairs	5′-GCATGCGTAATTTCAGTCGTAGTG (forward) 5′-GGCAAACTTTCTCCCACAAATG (reverse)	60 °C, 30 s	80.8
Rat NGFI-B mRNA primer pairs	5′-AAGATGCCGGTGACGTGCA (forward) 5′-CGGACTCTAGCAACAGGTCT (reverse)	60 °C, 30 s	80.5
Rat NR4A2 mRNA primer pairs	5′-AGTCTGATCAGTGCCCTCGT (forward) 5′-ATAGTCAGGGTTTGCCTGGAA (reverse)	60 °C, 30 s	81.7
Rat VP mRNA primer pairs	5′-TGCCTGCTACTTCCAGAACTGC (forward) 5′-AGGGGAGACACTGTCTCAGCTC (reverse)	60 °C, 30 s	80.7
Rat OT mRNA primer pairs	5′-AAGGCAGACTCAGGGTCG (forward) 5′-GGCATCTGCTGTAGCCCG (reverse)	60 °C, 30 s	84.5
Rat EGFP mRNA primer pairs	5′-GTGCCCATCCTGGTCGA (forward) 5′-CTTGCCGGTGGTGCAGA (reverse)	60 °C, 30 s	66.2
Human c-fos mRNA primer pairs	5′-TAGCCTCTCTTACTACCACTCA (forward) 5′-AGTGACCGTGGGAATGAAGTT (reverse)	60 °C, 30 s	84.2

T_A, annealing temperature and time; T_M, PCR product melting temperature.

^*Based on Kawasaki et al., 2009 and Ponzio et al., 2007.