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. 2016 Jul 27;12(10):1721–1737. doi: 10.1080/15548627.2016.1196316

Figure 2.

Figure 2.

GTP-bound Arl1 or Ypt6 are able to suppress the autophagy defect of the arl1Δ or ypt6Δ strains at 37°C. (A) GFP-Atg8 degradation was recovered when the GTP-restricted form of Arl1 was expressed in the arl1Δ strain. WT Arl1, empty vector (YEp352), N-terminal myristoylation defective Arl1 (G2A), GTP-bound Arl1 (Q72L), GDP-bound Arl1 (N127I), or nucleotide-free Arl1 (D130N) were transformed into the arl1Δ strain. The GFP-Atg8 processing assay was performed. (B) Pho8Δ60 activity was recovered when the GTP-restricted form of Arl1 was expressed in the arl1Δ (YSA003) strain. Error bars represent standard deviation from 3 biological replicates. The samples were done in 3 technical replicates for each biological replicate. (C) GFP-Atg8 degradation was recovered when the GTP-bound form of Ypt6 was expressed. Empty vector (pRS316), WT, GTP-bound Ypt6 (Q69L) or GDP-bound Ypt6 (T24N) were transformed into the ypt6Δ strain. The GFP-Atg8 processing assay was performed. (D) Pho8Δ60 activity was recovered when the GTP-bound form of Ypt6 was expressed in the ypt6Δ strain (YSA004). Error bars represent standard deviation from 3 biological replicates. The samples were done in 3 technical replicates for each biological replicate. EV, empty vector.