Figure 4.

Arl1 and Ypt6 are required in the formation of the autophagosome at 37°C. (A) Illustration of the GFP-Atg8 proteinase protection assay. (B) GFP-Atg8 proteinase protection assay was done as indicated in Materials and Methods. The arl1Δ and ypt6Δ strains were cultured in SD-N medium at 37°C, while the atg1Δ and ypt7Δ strains were at 30°C. The cartoon underneath represents different structures for autophagy in the presence of trypsin. The boomerang represents the phagophore. The double circles represent the autophagosome.