Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Jul 27;12(10):1721–1737. doi: 10.1080/15548627.2016.1196316

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 The Author(s). Published with license by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License http://creativecommons.org/licenses/by-nc/3.0/, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

PMC Copyright notice

Figure 7. — Arl1 and Ypt6 are required in the Cvt pathway. mRFP-Ape1 was integrated into the yeast genome using the pRS305-mRFP-Ape1 plasmid. Cells were grown and treated under starvation conditions as described. (A) Fluorescence images for WT (YSA005), atg1Δ (YSA006), arl1Δ (YSA007) and ypt6Δ (YSA008) strains in nonstarvation conditions. (B) The percentage of cells counted from Fig. 7A that contain a red prApe1 dot rather than the diffuse red phenotype. Error bars represent the standard deviation from 3 biological replicates. At least 80 cells were counted for each strain. (C) Fluorescence images for WT (YSA005), atg1Δ (YSA006), arl1Δ (YSA007) and ypt6Δ (YSA008) strains under starvation conditions at 30°C. (D) Fluorescence images for WT (YSA005), atg1Δ (YSA006), arl1Δ (YSA007) and ypt6Δ (YSA008) strains under starvation conditions at 37°C. (E) prApe1 processing assay for WT, atg1Δ, arl1Δ and ypt6Δ strains under nonstarvation conditions (time 0), starvation for 3 h at 30°C and starvation for 3 h at 37°C. N, nitrogen. Scale bar: 3 µm.