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. 2016 Jul 26;12(10):1791–1803. doi: 10.1080/15548627.2016.1203483

Figure 5.

Figure 5.

Effects of DUSP1 on RPS6KB, ULK1, and BECN1 phosphorylation. (A) Western blot analyses of the levels of total and phosphorylated RPS6KB (at Thr389) in Dusp1+/+ and dusp1−/− MEFs. Cells were left untreated or treated with 5 μM rapamycin for 20 h prior to being harvested. (B) Western blot analyses of the levels of p-ULK1 (at Ser555), ULK1, p-BECN1 (at Ser15) and BECN1 (upper panel), and quantification of p-ULK1 (Ser555) (middle panel) and p-BECN1 (Ser15) (lower panel). Dusp1+/+ and dusp1−/− MEFs were left untreated or treated with 5 μM rapamycin for 20 h prior to being harvested. (C) Western blot analyses of the levels of p-ULK1, ULK1, p-BECN1, BECN1 and MAPK1-MAPK3 in dusp1−/− MEFs (upper panel), and quantification of p-ULK1 (middle panel) and p-BECN1 (lower panel). Cells transfected with control siRNA (si-Control) or siRNA against Mapk1/3 (si-Mapk1/3) were left untreated or treated with 5 μM rapamycin for 20 h prior to being harvested. Data represent mean ± SD of 3 independent experiments. *, P < 0.01 and **, P < 0.005, statistically significant.