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. 2016 Oct 25;11(10):e0165466. doi: 10.1371/journal.pone.0165466

Fig 2. Brief priming of the revived cells with MSC-CM improves their proliferation and survival.

Fig 2

Cells frozen with CM and primed for 48 hrs with MSCs-CM (A) Flow chart depicting the experimental design.(B)Higher proliferation was seen in the cells primed with MSCs-CM.(C)The cell cycle analysis shows drastic reduction in the sub G0 phase with an increase in the percentage of cells in the S and G2/M phase in MSCs-CM set. (D)The histogram representation demonstrating the distribution of the cells in various stages of cell cycle. (E)Higher percentages of live CD34+ cells were found in the MSCs-CM as opposed to control. Data is represented as Mean ± standard deviation from 3 different independent experimental sets.