Fig 7. Sequence alignment of DGAT2 enzymes.

Dga1p-Yl: enzyme from Yarrowia lipolytica, Dga1p-Sc: Saccharomyces cerevisiae, DGAT2-Mm: Mus musculus. Alignment was performed using ClustalW [47]. Residues conserved in all three enzymes are on a black background, those conserved in two enzymes are on a grey background. TM domains are indicated in pink, as experimentally determined for Dga1p-Sc [39] and DGAT2-Mm [41]. TM domains predicted for Dga1p-Yl are yellow. The green background indicates the N-terminal residue fused to MBP in the recombinant soluble forms of Dga1p-Yl. The three regions highly conserved in DGAT2 enzymes and important for activity are emphasized up and down with blue rectangles.