Figure 1.

Growth in high glucose stimulates ECFC adhesion. A: ECFCs were grown in 25 mmol/L d-glucose or the l-glucose osmotic control media for 1 week, and a time course of their adhesion to an S17 cell monolayer was determined. n = 4. *P < 0.05. B: Cells grown for 1 week in the indicated media were harvested, and SDF-1α–stimulated migration was analyzed in a transwell assay. C: ECFCs express the α4-integrin. Solid line, isotype control antibody; dashed line, α4-specific antibody. D: Cells grown for 1 week in the indicated media were harvested and allowed to adhere for 30 min in the absence or presence of an anti-α4β1 blocking antibody (PS/2) or a nonspecific antibody (NS). n = 5. *P < 0.05.